Translational_Unit

Part:BBa_K2278021:Design

Designed by: Paul ZANONI   Group: iGEM17_INSA-UPS_France   (2017-10-08)


D-NY15 Antimicrobial peptide with Alpha-Factor Secretion Signal


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 244
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Part:BBa_K431009 : glyceraldehyde 3-phosphate dehydrogenase promoter (pGAP)

Part:BBa_K1800001:Design Alpha-Factor Secretion Signal


Source

The peptides DNA sequence has been obtained by reverse translation of the amino acid sequence of the D-NY15 proposed by Yaraksa et al., 2013, which was determined by mass spectrometry analysis.


References

Yaraksa, N., Anunthawan, T., Theansungnoen, T., Daduang, S., Araki, T., Dhiravisit, A. and Thammasirirak, S. (2013). Design and synthesis of cationic antibacterial peptide based on Leucrocin I sequence, antibacterial peptide from crocodile (Crocodylus siamensis) white blood cell extracts. The Journal of Antibiotics, 67(3), pp.205-212.